Abstract
We demonstrate the association of third-harmonic generation (THG) microscopy and particle image velocimetry (PIV) analysis as a novel functional imaging technique for automated micrometer-scale characterization of morphogenetic movements in developing embryos. Using a combined two-photon-excited fluorescence and THG microscope, we characterize the optical properties of Drosophila embryos and show that sustained THG imaging does not perturb sensitive developmental dynamics. Velocimetric THG imaging provides a quantitative description of the dynamics of internal structures in unstained wild-type and mutant embryos.
© 2004 Optical Society of America
Full Article | PDF ArticleMore Like This
Pierre Mahou, Nicolas Olivier, Guillaume Labroille, Louise Duloquin, Jean-Marc Sintes, Nadine Peyriéras, Renaud Legouis, Delphine Débarre, and Emmanuel Beaurepaire
Biomed. Opt. Express 2(10) 2837-2849 (2011)
Delphine Débarre, Willy Supatto, and Emmanuel Beaurepaire
Opt. Lett. 30(16) 2134-2136 (2005)
Shi-Wei Chu, Szu-Yu Chen, Tsung-Han Tsai, Tzu-Ming Liu, Cheng-Yung Lin, Huai-Jen Tsai, and Chi-Kuang Sun
Opt. Express 11(23) 3093-3099 (2003)