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  • 2019 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference
  • OSA Technical Digest (Optica Publishing Group, 2019),
  • paper cl_p_22

Fast multiphoton microscope based on polymer ulenses array using a 3D printed mold

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Multiphoton microscopy is a well-established technique for in vivo tissue imaging at high resolution and long penetration depth. As it uses a single point detector and a single scanning beam, image generation is slow. Several competitive solutions have been proposed during the last years, e.g. resonant scanning, that can reach 30 fps at 2048×2048 scanning resolution but relying on a fixed speed but it does not grant enough time for efficient collection of photons, or multiplexing beams [1,2], which show poor efficiency. Here, we propose a multiplexed beam solution that uses a rotating ulenses array for excitation and a camera for detection. The array of ulenses was built in polydimethilsiloxane (PDMS; > 90% transmission in the near IR wavelengths typically used in multiphoton microscopy), using a 3D-printed mold designed following a Nipkow disc pattern slightly modified to increase the lenses efficiency.

© 2019 IEEE

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