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Fluorescence lifetime optical projection tomography and FRET applied to visualizing apoptosis in live zebrafish larvae

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Abstract

We present the application of FLIM-OPT to read out biological function in live transgenic zebrafish larvae using a genetically expressed cleavable FRET biosensor for Caspase-3 as an indicator of gamma radiation induced apoptosis.

© 2016 Optical Society of America

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