Abstract

To date, fluorescence imaging systems have all relied on at least one beam splitter (BS) to ensure the separation of excitation light and fluorescence. Here, we reported SiO₂/TiO₂ multi-layer long pass filter integrated GaN LED. It is considered as the potential source for imaging systems. Experimental results indicate that the GaN LED shows blue emission peaked at 470.3 nm and can be used to excite dye materials. Integrating with a long pass filter (550 nm), the light source can be used to establish a real-time fluorescence detection for dyes that emit light above 550 nm. More interestingly, with this source, a real-time imaging system with signature words written with the dyes, such as ‘N J U P T’, can be converted into CCD images. This work may lead to a new strategy for integrating light sources and BS mirrors to build mini and smart fluorescence imaging systems.

© 2023 Chinese Laser Press

Multi-color fluorescence imaging based on plasmonic wavelength selection and double illumination by white light

Jong Min Choi, Kyungsik Eom, Seoyoung Hwang, Yena Lee, Sang Beom Jun, Kyung Min Byun, and Sung June Kim
Opt. Express 22(5) 5977-5985 (2014)

Structured illumination microscopy with partially coherent illumination for phase and fluorescent imaging

Kai Wen, ZhaoLin Gao, Xiang Fang, Min Liu, JuanJuan Zheng, Ying Ma, Zeev Zalevsky, and Peng Gao
Opt. Express 29(21) 33679-33693 (2021)

Digital micromirror device as a spatial illuminator for fluorescence lifetime and hyperspectral imaging

Artur Bednarkiewicz, Mounir Bouhifd, and Maurice P. Whelan
Appl. Opt. 47(9) 1193-1199 (2008)

Flat-field illumination for quantitative fluorescence imaging

Ian Khaw, Benjamin Croop, Jialei Tang, Anna Möhl, Ulrike Fuchs, and Kyu Young Han
Opt. Express 26(12) 15276-15288 (2018)

Angle-insensitive amorphous silicon optical filter for fluorescence contact imaging

Efthymios P. Papageorgiou, Hui Zhang, Bernhard E. Boser, Catherine Park, and Mekhail Anwar
Opt. Lett. 43(3) 354-357 (2018)