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THREE-DIMENSIONAL INTEGRAL MICROSCOPY WITH ENHANCED RESOLUTION AND DEPTH OF FIELD

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Abstract

Integral-imaging has demonstrated, in macroscopic and also in microscopy imaging, its capability for computing three-dimensional images from the microimages recorded after a single shot. The main drawback, however, of integral-imaging concept is the substantial reduction of the spatial resolution. In this contribution we explain two techniques, which permit to increase the two-dimensional spatial resolution of the computed depth images in integral microscopy. One technique is based in the physical interpolation in the microlens array structure. This is made by a double-shot approach, carried out by means of a rotating glass plate, which shifts the microimages in the sensor plane. The second method is based in the direct capture of the plenoptics map by placing a collection of microcameras at the aperture stop of the microscope objective. The second technique produces, also, a significant improvement of the depth of field.

© 2016 Optical Society of America

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