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Conferences > 3D > 2020 > DTh4A > DTh4A.3

Plant Cell Observation by TIE-based Fluorescence Imaging

Sudheesh K Rajput, Osamu Matoba, Manoj Kumar, Xiangyu Quan, Yasuhiro Awatsuji, and Yosuke Tamada

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Abstract

We present fluorescence imaging of plant cells based on transport of intensity equation and Fresnel propagation. At first, the phase distribution is obtained from the recorded three- defocus fluorescence intensity images and then from phase and corresponding intensity defocus image, the focus images of plant cell sample can be retrieved at various planes after free space propagation.

© 2020 The Author(s)

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