Quantitative Measurement of Fluorescence Resonance Energy Transfer (FRET) Efficiency

Tongsheng Chen

doi:10.1364/ACPC.2012.ATh1E.5

Asia Communications and Photonics Conference
OSA Technical Digest (online) (Optica Publishing Group, 2012),
paper ATh1E.5
•https://doi.org/10.1364/ACPC.2012.ATh1E.5

Quantitative Measurement of Fluorescence Resonance Energy Transfer (FRET) Efficiency

Tongsheng Chen

Asia Communications and Photonics Conference 2012

Guangzhou China
7–10 November 2012
ISBN: 978-1-55752-957-2

From the session
Micromanipulation and Cellular Microscopy (ATh1E)

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T. Chen, "Quantitative Measurement of Fluorescence Resonance Energy Transfer (FRET) Efficiency," in Asia Communications and Photonics Conference, OSA Technical Digest (online) (Optica Publishing Group, 2012), paper ATh1E.5.

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Abstract

We present some quantitative FRET methods based on partial acceptor photobleaching, termed emp-PbFRET. These methods can be used to determine the FRET efficiency of construct with multiple acceptors.

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