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Conferences > AOIPM > 1994 > OPTTM > OPTTM.278

Near-Infrared Optical Spectroscopy of Tissues Using an LED Frequency Domain Spectrometer

Enrico Gratton, Sergio Fantini, Maria Angela Franceschini, Joshua B. Fishkin, and John S. Maier

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Abstract

We present a light emitting diode (LED) based spectroscopic method for application in highly scattering materials. Using an LED as a light source, frequency domain measurement and the diffusion model of photon migration, we measured the absorption spectrum of a chromophore (methylene blue) suspended in a highly scattering medium (1.54% solids content Liposyn fat emulsion). The resulting spectrum agrees both qualitatively and quantitatively with standard spectropho-tometric measurements obtained without the scattering. Finally, we enumerate reasons why LED-based frequency-domain techniques are very appropriate for medical applications.

© 1994 Optical Society of America

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