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Advantages of fluorescence-mediated tomography: a prelude to molecular interrogations in deep tissues

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Abstract

We present certain experimental advantages of fluorescence measurements versus intrinsic contrast measurements pertaining to imaging the distribution of fluorochromes in tissue by means of a normalized Born expansion. Based on these observations we derive an appropriate reconstruction algorithm that concurrently uses intrinsic and fluorescence contrast that facilitates fluorescence-mediated molecular tomography in tissues in-vivo. In contrast to the standard Born approximation, the proposed algorithm does not require instrument calibration or absolute photon fluence measurements. Therefore it is ideally suited for experimental tomographic investigations of tissue in the near-infrared region. We have used this algorithm to image and quantify cancer associated cathepsin B expression in cancer animal models.

© 2002 Optical Society of America

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