Abstract

Two examples of the use of voltage sensitive dyes in Neurobiology will be presented. In the first, a single neuron is stained by intracellular injection and measurements of membrane potential in the cell body and in the dendritic tree are used to study the propagation of action potentials and synaptic potentials in this complex structure. In the second example, the olfactory bulb of a turtle is stained by superfusing a concentrated solution of the dye over the dorsal surface of the bulb. In this case all of the neurons, processes, and glia are stained and single neuron resolution cannot be obtained. The measurements are population signals. The turtle bulb responds to a presentation of odorant with three oscillations that differ in their latency, duration, frequency, and location.

© 2002 Optical Society of America