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Determination of VIS- NIR absorption coefficients of mammalian fat, with time- and spatially resolved diffuse reflectance and transmission spectroscopy

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Abstract

The objective is derived from the need for a reliable absorption spectrum of lipids for component analysis of in vivo tissue spectra. NIR in vivo spectroscopy enables to derive the concentration of the key tissue constituents absorbing in the 600-1100 nm range, that is oxy- and deoxy-hemoglobin, water and lipids. Yet, although the first three constituents are already well characterized in literature, quite few data are available on mammalian lipids. In the present proceeding we report the absorption spectrum of a clear purified oil obtained from pig lard. Absorption coefficients were measured with time- and spatially resolved diffuse reflectance spectroscopy techniques. At temperatures of 37°C and higher it is a clear transparent liquid thus suitable for collimated transmission measurements. In total three independent measurement techniques were employed to determine the absorption coefficients of mammalian lipids.

© 2004 Optical Society of America

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