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Two-Photon Imaging for the Non-Invasive Assessment of Electric Field Effects on Osteogenic Stem Cell Differentiation

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We present the use of two-photon excited fluorescence and second harmonic generation, to monitor electric field effects on osteogenic differentiation of human mesenchymal stem cells in terms of cellular morphology, biochemical composition and collagen deposition.

© 2008 Optical Society of America

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