Imaging RNA in Single Living Cells: Recent Advances and Future Outlook

Xuemei Zhang; Antony Chen; Andrew Tsourkas

doi:10.1364/BIOMED.2012.BM2A.2

Biomedical Optics and 3-D Imaging
OSA Technical Digest (Optica Publishing Group, 2012),
paper BM2A.2
•https://doi.org/10.1364/BIOMED.2012.BM2A.2

Imaging RNA in Single Living Cells: Recent Advances and Future Outlook

Xuemei Zhang, Antony Chen, and Andrew Tsourkas

Biomedical Optics 2012

Miami, Florida United States
28 April–2 May 2012
ISBN: 978-1-55752-942-8

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BioNanophotonics and Molecular Probes (BM2A)

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X. Zhang, A. Chen, and A. Tsourkas, "Imaging RNA in Single Living Cells: Recent Advances and Future Outlook," in Biomedical Optics and 3-D Imaging, OSA Technical Digest (Optica Publishing Group, 2012), paper BM2A.2.

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Abstract

Recently, we developed an oligonucleotide-based probe for imaging RNA in living cells, i.e. Ratiometric BiMolecular Beacons (RBMBs). RBMBs are highly sensitive, exhibit little/no false-positives, and can be used for prolonged imaging of single RNA transcripts.

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