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Conferences > BIOMED > 2012 > BM4B > BM4B.3

3D Super-resolution Imaging of Microtubules with a Double Helix Point Spread Function Microscope

Ginni Grover, Keith DeLuca, Sean Quirin, Jennifer DeLuca, and Rafael Piestun

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Abstract

Double Helix PSF Imaging of PtK1 cells reveals 3D intracellular structure beyond the diffraction limit. The 3D super-resolution microscope is built around a photon-efficient PSF design and phase mask matched to an optimal estimation algorithm.

© 2012 Optical Society of America

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