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Conferences > BIOMED > 2014 > BM3A > BM3A.53

Multi-frequency reference-free fluorescence lifetime imaging using a time-of-flight camera

Christopher Barsi, Refael Whyte, Ayush Bhandari, Anshuman Das, Achuta Kadambi, Adrian A. Dorrington, and Ramesh Raskar

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Abstract

Multi-frequency fluorescence lifetime imaging (FLI) has been demonstrated using a time-of-flight camera. Numerical fitting reveals the lifetime and depth of a fluorescent sample without the need of calibration or reference measurements. The method has potential use for decomposing multi-exponential decays in an affordable setup.

© 2014 Optical Society of America

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