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In vivo imaging of activity of targeted cell populations in the mouse olfactory bulb

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Abstract

Genetically encoded voltage indicators can be used to optically monitor electrical activity from genetically distinct populations of neurons. The FP voltage sensor ArcLight has been used to study intact neural circuits in Drosophila and C. elegans, and most recently, the mouse olfactory bulb. The goal of the present study was to genetically target ArcLight to mitral/tufted cells in the mouse olfactory bulb, and measure their population response to odorant presentation. A floxed version of the ArcLight AAV1 vector was developed, and was injected into Pcdh21-Cre transgenic mice, which resulted in expression restricted to mitral/tufted cells. ArcLight signals were measured using widefield epifluorescence imaging in anesthetized mice in response to odorants. ArcLight had sufficient signal size and temporal resolution to resolve the spatio-temporal activity elicited by individual consecutive sniffs of an odorant in single trials. ArcLight can be genetically targeted to specific cell types using Cre/LoxP recombination and can report population activity elicited by individual sniffs of an odorant.

© 2015 Optical Society of America

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