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Rapid Analysis of Fluorescence Quenching of FITC Conjugated Antibodies on Individual Cells by Phase-Sensitive Flow Cytometry

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Abstract

We demonstrate a high speed method to analyze the impact of self-quenching on fluorescence lifetimes in individual cells by phase-sensitive flow cytometry, using a model system consisting of FITC (fluorescein isothiocyanate) labeled antimouse Thy 1.2 antibodies bound to murine thymus cells.

© 1996 Optical Society of America

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