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Conferences > BRAIN > 2017 > BrM4B > BrM4B.5

In vivo multiphoton microscopy beyond 1 mm in the brain

David R. Miller, Flor A. Medina, Ahmed Hassan, Evan P. Perillo, Kristen Hagan, S.M. Shams Kazmi, and Andrew K. Dunn

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Abstract

We perform in vivo deep-tissue imaging of the mouse neocortex using multi-photon microscopy with a high repetition rate optical parametric amplifier laser source. We demonstrate an imaging depth of 1,200 µm in vasculature and 1,160 µm in neurons.

© 2017 Optical Society of America

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