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Imaging neuronal responses through all cortical layers and subplate of visual cortex in awake mice with optimized three-photon microscopy

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Abstract

In visual cortex, information is processed in multiple cortical layers. However, response features of neurons in deeper layers have remained unclear. Here, we design of a custom-made three-photon microscope to image a vertical column of the cerebral cortex >1 mm in depth in awake mice.

© 2019 The Author(s)

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