High-resolution structural and functional deep brain imaging using adaptive optics three-photon microscopy

Lina Streich; Juan Boffi; Ling Wang; Khaleel Alhalaseh; Matteo Barbieri; Ronja Rehm; Senthilkumar Deivasigamani; Cornelius Gross; Amit Agarwal; Robert Prevedel

doi:10.1364/BRAIN.2021.BM1B.3

Biophotonics Congress 2021
OSA Technical Digest (Optica Publishing Group, 2021),
paper BM1B.3
•https://doi.org/10.1364/BRAIN.2021.BM1B.3

High-resolution structural and functional deep brain imaging using adaptive optics three-photon microscopy

Lina Streich, Juan Boffi, Ling Wang, Khaleel Alhalaseh, Matteo Barbieri, Ronja Rehm, Senthilkumar Deivasigamani, Cornelius Gross, Amit Agarwal, and Robert Prevedel

Optics and the Brain 2021

Washington, DC United States
12–16 April 2021
ISBN: 978-1-943580-85-9

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Shaping Light in Space and Time (BM1B)

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L. Streich, J. Boffi, L. Wang, K. Alhalaseh, M. Barbieri, R. Rehm, S. Deivasigamani, C. Gross, A. Agarwal, and R. Prevedel, "High-resolution structural and functional deep brain imaging using adaptive optics three-photon microscopy," in Biophotonics Congress 2021, C. Boudoux, K. Maitland, C. Hendon, M. Wojtkowski, K. Quinn, M. Schanne-Klein, N. Durr, D. Elson, F. Cichos, L. Oddershede, V. Emiliani, O. Maragò, S. Nic Chormaic, N. Pégard, S. Gibbs, S. Vinogradov, M. Niedre, K. Samkoe, A. Devor, D. Peterka, P. Blinder, and E. Buckley, eds., OSA Technical Digest (Optica Publishing Group, 2021), paper BM1B.3.

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Abstract

We present an intravital imaging system based on three-photon excitation, indirect adaptive optics and active electrocardiogram gating which achieves near-diffraction-limited resolution up to 1.2mm in the mouse brain. We demonstrate the capability of our technique to study spine morphology and neuroglia function non-invasively throughout an entire cortical column and in the hippocampus in the mouse brain in-vivo.

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