Fourier-Based Quantification of Cerebral Oxygen Pressure Using Phosphorescence Lifetime Microscopy

Chang Liu; Chang Liu; Amanda Chisholm; Amanda Chisholm; Buyin Fu; Ikbal Sencan; Sava Sakadžić; Mohammad Abbas Yaseen; Mohammad Abbas Yaseen

doi:10.1364/BRAIN.2021.BTu1B.4

Biophotonics Congress 2021
OSA Technical Digest (Optica Publishing Group, 2021),
paper BTu1B.4
•https://doi.org/10.1364/BRAIN.2021.BTu1B.4

Fourier-Based Quantification of Cerebral Oxygen Pressure Using Phosphorescence Lifetime Microscopy

Chang Liu, Amanda Chisholm, Buyin Fu, Ikbal Sencan, Sava Sakadžić, and Mohammad Abbas Yaseen

Optics and the Brain 2021

Washington, DC United States
12–16 April 2021
ISBN: 978-1-943580-85-9

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Computational Methods for Estimation of Tissue Optical Properties, De-noising and Data Analysis (BTu1B)

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C. Liu, A. Chisholm, B. Fu, I. Sencan, S. Sakadžić, and M. A. Yaseen, "Fourier-Based Quantification of Cerebral Oxygen Pressure Using Phosphorescence Lifetime Microscopy," in Biophotonics Congress 2021, C. Boudoux, K. Maitland, C. Hendon, M. Wojtkowski, K. Quinn, M. Schanne-Klein, N. Durr, D. Elson, F. Cichos, L. Oddershede, V. Emiliani, O. Maragò, S. Nic Chormaic, N. Pégard, S. Gibbs, S. Vinogradov, M. Niedre, K. Samkoe, A. Devor, D. Peterka, P. Blinder, and E. Buckley, eds., OSA Technical Digest (Optica Publishing Group, 2021), paper BTu1B.4.

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Abstract

Phasor analysis provides a simple, Fourier-based method to visualize fluorescence lifetime. Here, we apply the technique for analyzing phosphorescence lifetime measurements and quantify oxygen tension in the cortical microvasculature in awake mice.

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