A flexible two-photon endoscope for fast activity imaging and cell-precise optogenetic photo-stimulation of neurons in freely moving animals

Nicolò Accanto; François G. C. Blot; Antonio Lorca Camara; Valeria Zampini; Florence Bui; Christophe Tourain; Noam Badt; Ori Katz; Valentina Emiliani

doi:10.1364/BRAIN.2022.BW4C.6

Biophotonics Congress: Biomedical Optics 2022 (Translational, Microscopy, OCT, OTS, BRAIN)
Technical Digest Series (Optica Publishing Group, 2022),
paper BW4C.6
•https://doi.org/10.1364/BRAIN.2022.BW4C.6

A flexible two-photon endoscope for fast activity imaging and cell-precise optogenetic photo-stimulation of neurons in freely moving animals

Nicolò Accanto, François G. C. Blot, Antonio Lorca Camara, Valeria Zampini, Florence Bui, Christophe Tourain, Noam Badt, Ori Katz, and Valentina Emiliani

Optics and the Brain 2022

Fort Lauderdale, Florida United States
24–27 April 2022
ISBN: 978-1-957171-03-6

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Neuronal Modulation, Optogenics and Wavefront Engineering (BW4C)

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N. Accanto, F. G. C. Blot, A. L. Camara, V. Zampini, F. Bui, C. Tourain, N. Badt, O. Katz, and V. Emiliani, "A flexible two-photon endoscope for fast activity imaging and cell-precise optogenetic photo-stimulation of neurons in freely moving animals," in Biophotonics Congress: Biomedical Optics 2022 (Translational, Microscopy, OCT, OTS, BRAIN), Technical Digest Series (Optica Publishing Group, 2022), paper BW4C.6.

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Abstract

We present a new micro-endoscope based on a fiber bundle and a GRIN lens, capable of 2P holographic photostimulation of multiple neurons with single cell precision and fast (100 Hz) 2P calcium imaging on fields of view of 250 µm in freely moving mice.

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