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Conferences > BRAIN > 2023 > BW1B > BW1B.2

Optimization of Confocal Bessel Beam Light-sheet for Axonal Connectomics

Kevin T. Takasaki, Olga Gliko, Russel Torres, Pooja Balaram, Ayana Hellevik, Emily Turschak, Bryan MacLennan, Sam Kinn, Eric Perlman, and R. Clay Reid

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Abstract

Axonal connectomics entails tracing of dense axons over large brain volumes. Here, we describe a light-sheet imaging approach using scanned Bessel beam illumination and confocal slit detection and the results of simulation-guided optimization. We evaluate the performance of our approach for axon resolution and segmentation.

© 2023 The Author(s)

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