Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
  • Conference on Lasers and Electro-Optics
  • OSA Technical Digest (Optica Publishing Group, 1990),
  • paper CTUH97

Detection of oxidized lipoproteins in blood by laser-induced fluorescence

Not Accessible

Your library or personal account may give you access

Abstract

Excess free radical production is known to cause tissue injury in a number of contexts such as coronary reperfusion, atherosclerosis, and lipopolysaccharide-induced endothelial cell injury. Free radicals have proved to be difficult to measure in vivo due to their low concentrations and short lifetime. Because free radicals readily oxidize lipoproteins, we propose to assess free radical production using the degree of oxidation of circulating lipoproteins as an index. An advantage of using oxidized lipoproteins as an indicator of free radical activity is that their half-lives in plasma are extremely long (minutes to hours depending on the extent of oxidation). In addition oxidized low density lipoprotein (o-LDL) has been shown to be toxic to various cells1 and is believed to be present in atherosclerotic plaques.2,3 Its level in plasma therefore may itself be an Important determinant of tissue damage. It has been demonstrated that when LDL is oxidized in vitro, its fluorescence emission spectrum is significantly altered.4 We measured LDL and o-LDL spectra in a fluorimeter with an excitation wavelength of 365 nm.

© 1990 Optical Society of America

PDF Article
More Like This
Techniques for in vivo laser-induced autofluorescence diagnostics

ROBERT M. COTHREN, REBECCA RICHARDS-KORTUM, RICHARD RAVA, and MICHAEL S. FELD
CTUO1 Conference on Lasers and Electro-Optics (CLEO:S&I) 1990

Optical Characterization of Europium Chlortetracycline Complexes in the Presence of Oxidized Low Density Lipoproteins

Luciane dos Santos Teixeira, Andrea Moreira Monteiro, A. M. Figueiredo Neto, Nilson Dias Vieira, Magnus Gidlund, and Lilia Coronato Courrol
PDPTuJ1 Latin America Optics and Photonics Conference (LAOP) 2010

Permeation of Human Plasma Lipoproteins in Vascular Tissues

Kirill V. Larin
MT1C.4 Optical Molecular Probes, Imaging and Drug Delivery (OMP) 2013

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved