Abstract
Excess free radical production is known to cause tissue injury in a number of contexts such as coronary reperfusion, atherosclerosis, and lipopolysaccharide-induced endothelial cell injury. Free radicals have proved to be difficult to measure in vivo due to their low concentrations and short lifetime. Because free radicals readily oxidize lipoproteins, we propose to assess free radical production using the degree of oxidation of circulating lipoproteins as an index. An advantage of using oxidized lipoproteins as an indicator of free radical activity is that their half-lives in plasma are extremely long (minutes to hours depending on the extent of oxidation). In addition oxidized low density lipoprotein (o-LDL) has been shown to be toxic to various cells1 and is believed to be present in atherosclerotic plaques.2,3 Its level in plasma therefore may itself be an Important determinant of tissue damage. It has been demonstrated that when LDL is oxidized in vitro, its fluorescence emission spectrum is significantly altered.4 We measured LDL and o-LDL spectra in a fluorimeter with an excitation wavelength of 365 nm.
© 1990 Optical Society of America
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