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  • Conference on Lasers and Electro-Optics
  • OSA Technical Digest (Optica Publishing Group, 1993),
  • paper CTuG5

Conlocal fluorescence spectroscopy of turbid media

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Abstract

The potential of fluorescence spectroscopy for diagnosis of epithelial neoplasia has recently been demonstrated.1 Tissue fluorescence spectra result from a weighted average of contributions from various layers; depth of penetration is governed by tissue optics, ranging to hundreds of microns. The optical sectioning property of the confocal fluorescence microscope can. be used to collect fluorescence from a particular layer with depth resolution of less than a micron.2 We have designed and constructed a confocal fiber optic spectrometer (CFOS) that combines both these properties. Data obtained from dilute and scattering samples are presented. Implications for enhancing tissue diagnosis and studying light propagation are discussed.

© 1993 Optical Society of America

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