Abstract

Spectroscopic methods have been explored to study pathology in human tissue [1]. Deconvolution of spectra to yield concentrations of tissue components has proven difficult for two reasons: (1) human tissue is a scattering and absorbing medium, and (2) the spectra of many biological chromophores overlap. While a number of methods exist to predict fluorophore concentrations in turbid samples, a direct comparison of these methods has not been made.

© 1994 Optical Society of America