Abstract

We present a novel technique, based on phase-sensitive flow cytometric principles,^1,2 that probes the excited state lifetimes of fluorochrome-labeled cells in flow simultaneously at more than one modulation frequency. It allows one to distinguish, in real time, between single-exponential and nonexponential decays for each cell at a high speed (hundreds to thousands of cells per second).

© 1995 Optical Society of America