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Conferences > CLEO > 2001 > CPD > CPD25
  • Conference on Lasers and Electro-Optics
  • OSA Technical Digest (Optica Publishing Group, 2001),
  • paper CPD25

FRET Imaging by Picosecond TCSPC Laser Scanning Microscopy

Wolfgang Becker, Axel Bergmann, Klaus Benndorf, Christoph Biskup, and Thomas Zimmer

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Abstract

Summary: Recording of time-resolved FRET images was achieved by combining a confocal laser scanning microscope (Zeiss LSM-510 Axiovert), a 150 femtosecond Coherent Titanium Sapphire Laser and an advanced Time-Correlated Single Photon Counting (TCSPC) imaging technique developed by Becker & Hickl, Berlin. The principle of the SPC-730 TCSPC Imaging module is shown in fig. 1.

© 2001 Optical Society of America

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