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Conferences > CLEO > 2001 > CWL > CWL5
  • Conference on Lasers and Electro-Optics
  • OSA Technical Digest (Optica Publishing Group, 2001),
  • paper CWL5

The application of nonlinear Raman spectroscopy to provide molecular specificity in 3-D biological imaging

G.R. Holtom, B.D. Thrall, B.Y. Chin, and T.H. Risby

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Abstract

Coherent Anti-Stokes Raman Spectroscopy (CARS) has been applied to microscopic imaging1 and is similar in method and application to two-photon fluorescence microscopy. A Stokes-shifted laser source (an optical parametric amplifier operating at 250 KHz), in combination with the 200 fs pump laser, is tightly focused into the sample, with high peak power but with nanojoule pulse energy.

© 2001 Optical Society of America

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