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  • Conference on Lasers and Electro-Optics
  • OSA Technical Digest (Optica Publishing Group, 2002),
  • paper CWC5

Spectrally Resolved TCSPC FRET Imaging

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Abstract

The setup consists of an LSM-510-NLO microscope (Zeiss), a PML-16 16 channel PMT detector head with a polychromator and an SPC-730 time-correlated single photon counting (TCSPC) imaging module (Becker & Hickl). The principle of the TCSPC imaging technique is shown in fig. 1.

© 2002 Optical Society of America

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