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  • Conference on Lasers and Electro-Optics
  • OSA Technical Digest (Optica Publishing Group, 2002),
  • paper CWC6

Nanometer X-ray Microscopy of Labeled Live Biological Organisms with a Nanosecond Laser-plasma Source

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Abstract

One of the highest priorities of microbiology today is the need to visualize live microorganisms, such as single cells, bacteria, viruses, etc., with sufficient temporal and spatial resolution and specificity to understand their structural and functional dynamics. Protein-specific, fluorescence-labeling, or ‘molecular-tagging’ and laserscanning confocaJ-opticai-microscopy [LSCM] is currently used, but is limited in spatial resolution to ~200-300 nm.1 X-ray microscopy [XRM] has achieved much higher spatial resolution2, and x-ray labeling techniques have been used, with synchrotron sources3.

© 2002 Optical Society of America

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