All optical histology of brain tissue: Serial ablation and multiphoton imaging with femtosecond laser pulses

Philbert S. Tsai; Beth Friedman; Varda Lev-Ram; Varda Lev-Ram; Qing Xiong; Qing Xiong; Roger Y. Tsien; Roger Y. Tsien; David Kleinfeld; Agustin I. Ifarraguerri; Beverly D. Thompson; Jeff A. Squier

Conference on Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference
Technical Digest (Optica Publishing Group, 2003),
paper CMN3

All optical histology of brain tissue: Serial ablation and multiphoton imaging with femtosecond laser pulses

Philbert S. Tsai, Beth Friedman, Varda Lev-Ram, Qing Xiong, Roger Y. Tsien, David Kleinfeld, Agustin I. Ifarraguerri, Beverly D. Thompson, and Jeff A. Squier

Conference on Lasers and Electro-Optics 2003

Baltimore, Maryland United States
1–6 June 2003
ISBN: 1-55752-733-4

From the session
Optical Techniques for Medical Diagnosis (CMN)

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P. S. Tsai, B. Friedman, V. Lev-Ram, Q. Xiong, R. Y. Tsien, D. Kleinfeld, A. I. Ifarraguerri, B. D. Thompson, and J. A. Squier, "All optical histology of brain tissue: Serial ablation and multiphoton imaging with femtosecond laser pulses," in Conference on Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference, Technical Digest (Optica Publishing Group, 2003), paper CMN3.

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Abstract

We demonstrate the first use of femtosecond laser pulses for serial histology. Successive iterations of multiphoton imaging and ablation provide diffraction-limited volumetric data that is used to reconstruct the architectonics of labeled cells or microvasculature.

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