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Using Integrated Multiple Microscopies to Monitor the Kinetics of SYTO 16 Dye Uptake during the Germination of Single Bacterial Spores

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Abstract

We present a methodology that combines fluorescence microscopy, phase contrast microscopy and laser tweezers Raman spectroscopy to monitor the kinetics of uptake of the nucleic acid dye SYTO 16 during germination of individual bacterial spores.

© 2011 Optical Society of America

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