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Light Sheet Microscopy with Wavefront Coding for Fast Volumetric Imaging of Biological Samples

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Abstract

Decoupling the illumination from the detection optics in microscopy can be achieved by combining light sheet and wavefront coding. This results in a system that allows the light sheet to produce optical sections from out of focus planes. By scanning the light sheet through the sample, it is possible to produce high-resolution volumetric images of living samples at unprecedented speeds.

© 2016 Optical Society of America

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