Abstract

Recent studies of resonance energy transfer have extended the early works of Perrin and Galanin to include, not only the residual anisotropy following near zone Förster-type energy transfer, but also the retardation effects manifest in the long range radiative results [1,2]. In this paper we report new theoretical results for two photon fluorescence migration in a two-component system, determining the residual fluorescence anisotropy at both the long and short range asymptotes. In employing a two level approximation for the polar centre we are able to obtain tractable results for the fluorescence anisotropy ratios, in a form that resembles Perrin’s (single centre) result.

© 1996 IEEE