Abstract
Optical microscopy offers a wide variety of powerful imaging techniques at moderate costs and is compatible with almost all kinds of samples. The applicability is, however, limited by the contrast achievable. A highly desirable chemically selective contrast mechanism is vibrational spectroscopy. Such an approach can be pursued with direct infrared absorption microscopy, or confocal Raman microscopy. However, IR imaging suffers from low spatial resolution, and Raman microscopy from very weak signals. Employing the nonlinear coherent anti-Stokes Raman scattering (CARS) process instead of spontaneous Raman, high signal levels and thus short acquisition times can be achieved, if a multiplexed spectral acquisition is used.
© 2007 IEEE
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