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Optica Publishing Group
  • CLEO/Europe and EQEC 2009 Conference Digest
  • (Optica Publishing Group, 2009),
  • paper CL4_4

Surface Plasmon-Mediated Fluorescence Microscopy: a powerful imaging technique for membrane imaging

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Abstract

Total internal reflection fluorescence microscopy (TIRFM) employs the unique properties of an induced evanescent wave to selectively illuminate and excite fluorophores in a restricted specimen region immediately adjacent to the glass coverslip interface. TIRFM is employed to investigate the interaction of molecules with surfaces, an area that is of fundamental importance to a wide spectrum of disciplines in cell and molecular biology. Living cells in particular provide excellent candidates for TIRFM investigations to study membranes phenomena and adhesion interactions. Today, the technique is gaining popularity in part because new high numerical aperture microscope objective lenses have been developed (NA≥1.45).

© 2009 IEEE

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