Abstract
Total internal reflection fluorescence microscopy (TIRFM) employs the unique properties of an induced evanescent wave to selectively illuminate and excite fluorophores in a restricted specimen region immediately adjacent to the glass coverslip interface. TIRFM is employed to investigate the interaction of molecules with surfaces, an area that is of fundamental importance to a wide spectrum of disciplines in cell and molecular biology. Living cells in particular provide excellent candidates for TIRFM investigations to study membranes phenomena and adhesion interactions. Today, the technique is gaining popularity in part because new high numerical aperture microscope objective lenses have been developed (NA≥1.45).
© 2009 IEEE
PDF ArticleMore Like This
Karla Balaa, Viviane Devauges, Yannick Goulam, Vincent Studer, Sandrine Lévêque-Fort, and Emmanuel Fort
7367_10 European Conference on Biomedical Optics (ECBO) 2009
V. Devauges, P. Blandin, J. C. Cossec, S. Lécart, C. Marquer, M.C. Potier, F. Druon, P. Georges, and S. Lévêque-Fort
7367_11 European Conference on Biomedical Optics (ECBO) 2009
Herbert Schneckenburger, Michael Wagner, Petra Weber, Thomas Bruns, Heiko Steuer, and Brigitte Angres
7368_0Q European Conference on Biomedical Optics (ECBO) 2009