Abstract
Optical microscopy is the modern workhorse of biology, providing high contrast images, often in real time of biological processes at sub-cellular distance scales. Nevertheless, improved understanding of biological processes is difficult to achieve with diffraction-limited resolution as is offered by standard optical microscopy techniques. Sub-diffraction limited resolution has been achieved by two notable techniques: stimulated emission depletion (STED) microscopy [1], and stochastic optical reconstruction microscopy (STORM) [2], but a significant disadvantage of both approaches is that they require chemically attached fluorescent labels in the sample.
© 2009 IEEE
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