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Conferences > CLEO_Europe > 2013 > CL_4 > CL_4_2
  • 2013 Conference on Lasers and Electro-Optics - International Quantum Electronics Conference
  • (Optica Publishing Group, 2013),
  • paper CL_4_2

Interferometric Second Harmonic Generation microscopy for tissue imaging

M. Rivard, K. Popov, M. Laliberté, A. Bertrand-Grenier, F. Martin, H. Pépin, C. P. Pfeffer, C. Brown, L. Rammuno, and F. Légaré

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Abstract

Second Harmonic Generation (SHG) microscopy is used to image tissues rich in noncentrosymmetric proteins such as collagen [1]. Two identical noncentrosymmetric structures with opposite orientation will emit a π phase shifted SHG signal because their second order nonlinear susceptibility tensors (χ(2)) are inverted. This can be exploited to obtain additional information about the orientation of noncentrosymmetric structures in a sample by measuring the phase of the SHG signal generated in an area scanned with SHG microscopy.

© 2013 IEEE

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