Abstract
Coherent Raman Scattering (CRS) is an emerging label-free microscopy technique, due to its capability of identifying the chemical components in tissues and cells based on their characteristic vibrational response. The most powerful CRS technique is Stimulated Raman Scattering (SRS), which provides a signal that is linear with molecular concentration and free from non-resonant background [1]. Despite these important advantages, SRS is technologically very demanding, since it requires the measurement of a tiny differential signal sitting on top of a large and noisy background. CRS requires two narrowband, picosecond synchronized pulse trains with tunable frequency difference, high repetition rate (≈100 MHz) and output power >100 mW per branch. The golden standard for CRS imaging are Yb-laser pumped optical parametric oscillators (OPO), which naturally provide frequency detuning, but are often complex, costly, and, due to various degrees of freedom, difficult to control.
© 2015 IEEE
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