Abstract

Coherent anti-stokes Raman scattering (CARS) spectroscopy and microscope is used for investigating the chemical or biological dynamics in living samples without staining or fluorescent tags[1]. In order to obtain the CARS signal from samples, different two wavelengths and synchronization of pulses generated from individual lasers by using optical delay line achieved by mechanical moving, and also wavelength tuning are required. So the excitation light source and the signal include the jitter noises occurred when the wavelengths are tuned and the pulse timing is synchronized. The stability of laser intensity in excitation light source is most important and required factor to obtain the CARS spectrum with high signal to noise ratio and achieve quantitative measurements of samples.

© 2015 IEEE