Abstract

Fluorescence microscopy is invaluable in materials science and life sciences. Due to the diffraction limit conventional microscopy systems are not capable of resolving structures that are smaller than roughly 200 nm. A breakthrough in fluorescence microscopy was recently achieved by nonlinear optical phenomena for high-resolution imaging. With dyes with specific photophysics the diffraction limit can be circumvented to yield sub-200 nm resolution [1-3]. However, such dyes are not always suited for labeling the structures of interest.

© 2015 IEEE