Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
  • 2015 European Conference on Lasers and Electro-Optics - European Quantum Electronics Conference
  • (Optica Publishing Group, 2015),
  • paper PL_1_1

Far-field optical nanoscopy: principles and recent advancements

Not Accessible

Your library or personal account may give you access

Abstract

Throughout the 20th century it has been widely accepted that, at the end of the day, a light microscope relying on conventional lenses (far-field optics) cannot discern details that are finer than about half the wavelength of light (> 200 nm). However, in the 1990s, it was discovered that overcoming the diffraction barrier is realistic and that fluorescent samples can be resolved virtually down to molecular dimensions. Here we discuss the simple yet powerful principles that allow neutralizing the resolution-limiting role of far-field optical diffraction1,2. In a nutshell, features residing closer than the diffraction barrier are prepared in different molecular (quantum) states so that they are distinguishable for a brief detection period. As a result, the resolution-limiting role of diffraction is overcome, and the interior of transparent samples, such as living cells and tissues can now be imaged non-invasively at the nanoscale using focused light in 3D.

© 2015 IEEE

PDF Article
More Like This
Far-field optical nanoscopy

Stefan W. Hell
JSV1_3 European Quantum Electronics Conference (EQEC) 2009

FAR-FIELD OPTICAL NANOSCOPY

Stefan W. Hell
PL_3 Conference on Lasers and Electro-Optics/Pacific Rim (CLEOPR) 2009

Far-Field Optical Nanoscopy

Volker Westphal, Silvio O. Rizzoli, Marcel A. Lauterbach, Dirk Kamin, and Stefan W. Hell
NMA1 Novel Techniques in Microscopy (NTM) 2009

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2022 | Optica Publishing Group. All Rights Reserved