Abstract

Advances in the research fields of biological, biophysical and biochemistry rely on the development of novel, flexible, powerful and reliable laser sources in the visible – NIR part of the spectrum [1, 2]. Optical excitation in the 750-800 nm region, with flexible pulsed formats, can be advantageous in applications such as confocal fluorescence microscopy, STED, FLIM microscopy, photoluminescence spectroscopy, laser photocoagulation and time-resolved spectroscopy. Finely tailored pulse formatting can indeed provide significant enhancement in many of those techniques [3] by optimizing the temporal distribution of the optical excitation with respect to the specific characteristics of the fluorophore of interest such as its excited-state lifetime for example.

© 2017 IEEE