Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
  • 2019 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference
  • OSA Technical Digest (Optica Publishing Group, 2019),
  • paper ch_10_1

High-resolution, three-dimensional investigation of protein diffusion and trafficking in the live HeLa cell via interferometric scattering microscopy (iSCAT)

Not Accessible

Your library or personal account may give you access

Abstract

Transmembrane proteins exhibit a wide variety of mobility in live cells, including diffusion in the plasma membrane and directed transport into the cell via endocytosis. Although a great deal has been uncovered about the biochemistry underlying these processes, their real-time visualisation in situ with the necessary nanoscopic resolution remains a daunting experimental challenge. Conventional microscopies rely on fluorescent labelling to monitor diffusion of such proteins within live cells. The resolution in both space and time by which one can successfully determine the position of the protein is curtailed by the finite and limited emission rate of the fluorophore.

© 2019 IEEE

PDF Article
More Like This
Visualizing Cellular Secretion with Single-Protein Sensitivity via Interferometric Scattering Microscopy (iSCAT)

André Gemeinhardt, Katharina König, Francesca Nicoli, Mahyar Dahmardeh, and Vahid Sandoghdar
jsii_2_3 European Quantum Electronics Conference (EQEC) 2019

Label-Free Confocal iSCAT Microscopy on Live Cells

Michelle Küppers, David Albrecht, Anna Kashkanova, Jennifer Lühr, and Vahid Sandoghdar
NTh2C.5 Novel Techniques in Microscopy (NTM) 2021

Label-Free Live-Cell Imaging with Interferometric Scattering Microscopy: Confocal Imaging and High-Speed 3D Single Particle Tracking

Michelle Küppers, Anna Kashkanova, Jennifer Lühr, David Albrecht, Alexandra Schambony, and Vahid Sandoghdar
MW1A.4 Microscopy Histopathology and Analytics (Microscopy) 2020

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2022 | Optica Publishing Group. All Rights Reserved