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  • 2019 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference
  • OSA Technical Digest (Optica Publishing Group, 2019),
  • paper ch_13_4

Probing living cells by terahertz attenuated total reflection. Application to permeabilization dynamics

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Abstract

Using attenuated total reflection (ATR) in the terahertz domain [1], we demonstrate non-invasive, non-staining real time measurements of cytoplasm leakage during permeabilization of live epithelial cells. We show that terahertz modalities are more sensitive than fluorescence microscopy which is the reference optical technique for electropermeabilization. We also propose analytical models for the influx and efflux of non-permeant molecules through permeabilized cell membranes by electropermeabilization or by detergent.

© 2019 IEEE

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