Abstract

The application of advanced microscopy imaging techniques to 3D specimens or motions faces the problem of the limited depth of field of optical lenses [1]. Thanks to numerical focus computations, digital holography (DH) releases these limitations and extends significantly the allowed axial range of imaging. However, the digital cost of focus distance determination and of object reconstruction makes real-time 3D imaging hardly possible, especially when both in-plane and out-of-plane metrics must be extracted simultaneously.

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