Abstract

Fluorescence lifetime imaging (FLIM) is an indispensable tool for many fields, including material research or biophysics. Therefore, the search for a new robust and low-cost method for FLIM is of high interest. We present a novel RAndom Temporal Signals (RATS) method for FLIM. The RATS technique reaches a higher signal-to-noise ratio (SNR) compared to conventional time-resolved methods due to continuous random excitation. Moreover, it eliminates the need to choose the correct repetition pulse rate and avoids additional signal timing.

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