Abstract

The current extremely rapid development of biomedical imaging techniques, such as OCT and multiphoton microscopy, requires the development of light sources with very sophisticated parameters. We present a femtosecond laser emitting at the borderline of visible and near-infrared range (760-800 nm). The intended application is in two-photon excited fluorescence microscopy, in which a fluorophore simultaneously absorbs two photons from the excitation beam. Compared to traditional (single-photon) fluorescence microscopy, the two-photon variant allows deeper penetration into imaged tissue, lower phototoxicity, and guarantees confocality (without the need for an aperture) [1]. This technique makes it possible to image, for example, the retina of the eye [2]. The laser we propose is an all-fiber-based solution that is an alternative to the titanium-sapphire lasers currently in use, offering compactness so that it can be easily integrated into existing devices (such as microscopes or ophthalmoscopes).

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