Add to BibSonomyAbstract
We have developed a nonlinear spectral unmixing algorithm that separates fluorescence excitation-emission matrix of multiple fluorophores affected by the inner filter effect. We evaluate this technique on simulated data and demonstrate its superior performance experimentally for a mixture of fluorophores.
© 2014 Optical Society of America
PDF ArticleMore Like This
Improved sensitivity by applying spectral unmixing prior to fluorescent tomography
Heng Xu, Chaincy Kuo, and Brad Rice
BMC1 Biomedical Optics (BIOMED) 2008
Spectral unmixing of multi-color tissue specific in vivo fluorescence in mice
Giannis Zacharakis, Rosy Favicchio, Anikitos Garofalakis, Stylianos Psycharakis, Clio Mamalaki, and Jorge Ripoll
6626_8 European Conference on Biomedical Optics (ECBO) 2007
Non-Negative Matrix Factorization to unmix several fluorescence spectra and remove autofluorescence from in vivo spectrally resolved acquisitions
Anne-Sophie Montcuquet, Lionel Hervé, Fabrice Navarro, Jean-Marc Dinten, and Jérôme I. Mars
BSuD39 Biomedical Optics (BIOMED) 2010